Part:BBa_K3790085:Design

T7 phage gene product 2, an E.coli transcription inhibitor

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The following primers were used for constructing this part.

gp2_coding1 	5-GGGCTACCGTAGAGTCCTCGGAGCATTCCTTCGAGGTTCCAATCTACGCTGAaACCC
gp2_coding2	5-CAGGTTCACTTAGTGTGGACAATAAGAAGTTTTGGGCTACCGTAGAGTCCTC
gp2_coding3	5-ATGTCAAACGTAAATACAGGTTCACTTAGTGTGGACAATAAG
gp2_template1	5-GGAACGTATTGCCATTCGGCTAACTCCAGAGCTTCGTCTAGGGTtTCAGCGTAGATTGG
gp2_template2	5-CAAGGACGCACACGAGTAACCTCAAAGCCAGCCGGAACGTATTGCCATTCGGC
gp2_template3	5-TTACTTCGGTGCTACACAAGGACGCACACGAGTAAC
doubleter_f	5-CGATATAAGTTGTAATTCTCaaataataaaaaagccggattaataatctggc
doubleter_r	5-GAAGGCTCTCAAGGGCATtcacactggctcaccttcg
gp2_f		5-GAATGCCCTTGAGAGCCTTCTTACTTCGGTGCTACACAAGGAC
gp2_r		5-gagttaaggaggtaaaaaaaATGTCAAACGTAAATACAGGTTCACTTAG
pBAD_f		5-tttttttacctccttaactctagtagctagcc
pBAD_r		5-TGGTGCTACGCCTGAATAAGttatgacaacttgacggctacatcattc
pLysS_f		5-tagccgtcaagttgtcataaCTTATTCAGGCGTAGCACCAG
pLysS_r		5-GAGAATTACAACTTATATCGTATGGGGC